High SHG throughput for time resolved, label free imaging of neurones
© 2015 EPFL
We are very pleased to announce and kindly invite you to our monthly EPFL Photonics Chapter (EPC) 'Pizza-optics-beer' (POB) seminar on April 8th, 2015 (Wednesday) from 17h00 to 18h00. The seminar will take place in the room CM 012. This month Marie Didier will give us some insights in her field of expertise. Her presentation in the seminar is entitled "High Second harmonic generation throughput for time resolved, label free imaging of neuronal activity".
Abstract: We describe a wide-field second harmonic imaging method with high throughput that can be used to probe dynamics in living cells1 with image acquisition times of 50 microseconds. Low fluence imaging of cultured primary neurons is also possible2. We perform low fluence polarization resolved in-vitro imaging of living cultured mouse brain neurons to image real-time structural changes that can be associated to changes in the structural integrity and transport mediated by microtubules. SHG images and movies can be recorded not only from axons but also from dendrites and the somata and display a wide variety of dynamical changes.